Influence of pre-plant densities of Meloidogyne incognita on growth and root infestation of spinach (Spinacia oleracea L.) (Amaranthaceae) – an important dimension towards enhancing crop production

Dammini Premachandra, Simon Gowen


Vegetables represent a main source of micro-nutrients which can improve the health status of malnourished poor in the world. Spinach (Spinacia oleracea L.) is a popular leafy vegetable in many countries which is rich with several important micro-nutrients. Thus, consuming Spinach helps to overcome micro-nutrient deficiencies. Pests and pathogens act as major yield constraints in food production. Root-knot nematodes, Meloidogyne species, constitute a large group of highly destructive plant pests. Spinach is found to be highly susceptible for these nematode attacks. Though agricultural production has largely benefited from modern technologies and innovations, some important dimensions which can minimize the yield losses have been neglected by most of the growers. Pre-plant or initial nematode density in soil is a crucial biotic factor which is directly responsible for crop losses. Hence, information on pre-plant nematode densities and the corresponding damage is of vital importance to develop successful control procedures to enhance crop production. In the present study, effect of seven initial densities of M. incognita, i.e., 156, 312, 625, 1250, 2,500, 5,000 and 10,000 infective juveniles (IJs)/plant (equivalent to 1000cm3 soil) on the growth and root infestation on potted spinach plants was determined in a screen house. In order to ensure a high accuracy, root infestation was ascertained by the number of galls formed, the percentage galled-length of feeder roots and galled-feeder roots, and egg production, per plant. Fifty days post-inoculation, shoot length and weight, and root length were suppressed at the lowest IJs density. However, the pathogenic effect was pronounced at the highest density at which 43%, 46% and 45% reduction in shoot length and weight, and root length, respectively, was recorded. The highest reduction in root weight (26%) was detected at the second highest density. The Number of galls and percentage galled-length of feeder roots/per plant showed significant progressive increase across the increasing IJs density with the highest mean value of 432.3 and 54%, respectively. The two shoot growth parameters and root length showed significant inverse relationship with the increasing gall formation. Moreover, the shoot and root length were shown to be mutually dependent on each other. Suppression of shoot growth of spinach greatly affects the grower’s economy. Hence, control measures are essentially needed to ensure a better production of spinach via reducing the pre-plant density below the level of 0.156 IJs/cm3.

Key words: density; Meloidogyne incognita; shoot growth; spinach

Data of the article

First received: 13 August 2015 | Last revision received: 11 October 2015
Accepted: 03 November 2015 | Published online: 09 December 2015
URN: nbn:de:hebis:34-2015092949091

Full Text:



Anwar, S. A., & Din, G.M. (1986). Nematodes: Biotic constrains to plant health. Parasitology 3, 48-53.

Anwar, S. A., & Van Gundy, S.D. (1993). Effect of Meloidogyne incognita on root and shoot growth parameters of susceptible and resistant varieties of tomato. Afro-Asian Journal of Nematology 3, 152-160.

Azam, T., Hisamuddin, S. S., & Robab, M. I. (2011). Effect of different inoculum levels of Meloidogyne incognita on growth and yield of Lycopersicon esculentum, and internal structure of infected root. Archives of Phytopathology and Plant Protection 44, 1829–1839.

Barker, K. R., & Olthof, T. H. A. (1976). Relationships between nematode population densities and crop responses. Annual Review of Phytophatology 14, 327-353.

Castillo, P., & Jiménez-Díaz, R. M. (2003). First report of Meloidogyne incognita infecting spinach in Southern Spain. Phytopathology 87, p. 874 (abstract).

Castagnone-Sereno P., Bongiovanni, M., & Dalmasso, A. (1993). Stable virulence against tomato resistance Mi gene in the parthenogenetic root-knot nematode Meloidogyne incognita. Phytopathology 83, 803–805.

Charegani, H., Majzoob, S., Hamzehzarghan, H., & Karegar-Bide, A. (2012). Effect of various initial population densities of two species of Meloidogyne on growth of tomato and cucumber in greenhouse. Nematologia Meditereria 40, 129-134.

Eisenback , J. D., & Triantaphyllou, H.H. (1991). “Root-knot nematodes Meloidogyne sp. and races”. In: Nickle, W.R. (Ed.): Manual of agricultural nematology. pp. 191-274. Marcal Dekker, New York.

Ekanayake, H.M.R.K. (2001). Histopathological changes caused by Meloidogyne graminicola in rice roots. Annals of the Sri Lanka Department of Agriculture 3, 43-46.

Ekanayake, H. M. R. K., & Toida, Y. (1997). Nematode parasites of Agricultural Crops and their distribution in Sri Lanka. JIRCAS Journal 4, 23-39.

Ekanayake, H. M. R. K., Vito, M. D., & Vovlus, N. (1988). Histopathological cahnges caused by Meloidogyne incognita on tomato and egg plants roots. Tropical Agriculturist 144, 89-97.

Ferris, H. (1978). Nematode economic thresholds: derivation, requirements and theoretical consideration. Journal of Nematology 10, 341-350.

Ferris, H. (1985). Density-dependent nematode seasonal multiplication rates and over-winter survivorship: A critical point model. Journal of Nematology 17, 93-100.

Fortnum, B.A., Kasperbauer, M.J., Hunt, P.G., & Bridges, W.C. (1991). Biomass partitioning in tomato plants infected with Meloidogyne incognita. Journal of Nematology 23, 291-297.

Greco, N., & Di Vito, M. (2009). “Population dynamics and damage levels”. In R. N.Perry, M. Moens and J. L. Starr (Eds.). Root-knot Nematodes pp. 246 -274. CAB International, Wallingford, UK.

Griffin G.D. (1981). The relationship of plant age, soil temperature, and population density of Heterodera schachtii on the growth of sugarbeet. Journal of Nematology 13, 184-190.

Hussey, R. S., & Grundler, F. M. W. (1998). “Nematode parasitism of plants”. In:. Perry, R. N., & Wright, D. J. (Eds.), The Physiology and Biochemistry of free-living and plant-parasitic nematode spp. 213-243. CAB International, Wallingford, UK.

Kankam, F. , & Adomako, J. (2014). Influence of inoculum Levels of Root Knot Nematodes (Meloidogyne spp.) on Tomato (Solanum lycopersicum L.). Asian Journal of Agriculture and Food Science 2, 171-178.

Khan, T. A., Ashraf, M. S., & Hasan, S. (2006). Pathogenicity and life cycle of Meloidogyne javanica on balsam (Impatiens balsamina). Archives of Phytopathology and Plant Protection 39, 45-48.

Kinloch, R.A. (1982). The relationship between soil populations of Meloidogyne incognita and yield reduction of soybean in the coastal plain. Journal of Nematology 14, 162-167.

Korayem, A. M. (2006). Relationship between Meloidogyne incognita density and damage to sugar beet in sandy clay soil. Egypt Journal of Phytophathology 34, 61-68.

Madamba, C. P., Sasser, J. N., & Nelson, L. A. (1965). Some characteristics of the effects of Meloidogyne spp. on unsuitable host crops. North Carolina Agricultural Experimental Station Technical Bulletin 169, 1-34.

Mekete, T., Mandefro, W., Greco, N. (2003). Relationship Between initial population densities of Meloidogyne javanica and Damage to Pepper and Tomato in Ethiopia. Nematologia Mediterranea 31, 169-171.

Olthof, T. H. A., & Potter, J. W. (1972). Relationship between population densities of Meloidogyne hapla and crop losses in summer maturing vegetables in Ontario. Phytopathology 62, 981-986.

Olthof, H. A., & Potter, J. W. (1977). Effects of population dynamics of Meloidogyne hapla on growth and yield of tomato. Journal of Nematology 4, 296-300.

Pang, W., Hafez, S. L., & Sundararaj, P. (2009). Pathogenicity of Meloidogyne hapla on onion. Nematotropica 39, 225-233.

Pankaj Sharma, Mishra., S. D., and Kamra, A. (2001). Integrated management of the root-knot nematode Meloidogyne incognita in spinach (Spinacia oleracea L.). Indian Journal of Nematology 31, 165–166.

Patel, M. B., Patel, D. J., & Patel, B. A. (1996). Pathogenic effects of Meloidogyne incognita and M. javanica on cotton. Afro-Asian Journal of Nematology 6, 156-161.

Potter, J. W., & Olthof, T. H A. (1993). “Nematode pest of vegetable crops”. In: Evans, K., Trudgill, D. L., & Webster, J. M, (Eds): Plant Parasitic Nematodes in Temperate Agriculture. pp. 171–207. CAB International Wallingford, UK.

Potter, J. W., & Olthof, T. H. A. (1974). Yield losses in fall-maturing vegetables relative to population densities of Pratylenchus penetrans and Meloidogyne hapla. Phytopathology 64, 1072–1075.

Premachandra, W. T. S. D., Lasanthi, A. H. P., Deepananda, K. H. M.A., & Jayasinghe, R. C. (2006). A preliminary study on root-knot nematodes, Meloidogyne species and their bacterial hyper-parasite, Pasteuria penetrans associated with Spinach in Matara District. Proceedings of Third Academic Sessions, University of Ruhuna, Matara, Sri Lanka. pp. 127-131.

Premachandra, W. T .S. Dammini., & Senarath, D. P. C. (2011). Root-knot nematode infestations on Chilli (Capsicum annuum) at selected localities in Hambanthota district. Proceedings of the 30th annual sessions of Institute of Biology, Sri Lanka, p. 26 (abstract).

SAS Institute (1999). SAS/STAT user’s guide. SAS Institute, Cary, NC.

Sasanelli, N. (1994). Tables of nematode pathogenicity. Nematologia Mediterranea 22, 153–7.

Sasser, J. N., & Freckman, D. W. (1987). “A world perspective on nematology: the role of the society”. In: Veech, J.A., & Dickson, D.W. (Eds.): Vistae on Nematology: a commemoration of the Twenty-fifth Anniversary of the Society of Nematologists, Inc. pp. 7-14, Hyattsville.

Schomaker, C.H., & Been, T.H. (2006). “Plant growth and population dynamics”. In: Perry, R., & Moens, M (Eds.): Plant nematology. pp. 275-295. CAB International Wallingford, UK.

Shahab, S.S., & Sharma, S. (2011). Pathogenicity of root-knot nematode, Meloidogyne incognita and root rot fungus, Rhizoctonia solani on okra (Abelmoshcus esculentus L.). Journal of Science and Technology 3, 97-102.

Sikora, R. A., & Fernandez, E. (2005). ”Nematode parasites of vegetables”. In: Luc, M., Sikora, R. A., & Bridge, J. (Eds.): Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, second ed. pp. 319-392. CABI Publishing, Wallingford, UK.

Szalay, Jessie. (2015). Spinach: Health Benefits, Nutrition Facts (& Popeye). Retrieved from on 12th August 2015.

Taylor, A. L., & Sasser, J. N. (1978). Biology, Identification and Control of Root-Knot Nematode, Meloidogyne spp. A ccoperative publication of the Department of Plant Pathology, North Carolina State University and U.S. Agency for International Development. p. 111.

Vito, M. D., Vovlas, N., & Castillo, P. (2004). Host-parasitic relationships of Meloidogyne incognita on spinach. Plant pathology 53, 508-514.

Williamson, V. M., & Hussey, R. S. (1996). Nematode pathogenesis and resistance in plants. Plant Cell 8, 1735-1745.

Zahid, M. I., Nobbs, J., Geoff, M. G., Hodda, M., Alexander, N., William, J.F., & Nicol, H. I. (2001). Effect of the clover root-knot nematode (Meloidogyne trifoliophila) on growth of white clover. Nematology 3, 437-446.


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